Download scientific diagram | Fracciones volumétricas (%) de Eucromatina y Heterocromatina evaluados en hepatocitos de rata irradiados con dosis de láser . Download scientific diagram| Fracciones volumétricas (%) de Eucromatina y Heterocromatina evaluados en hepatocitos de rata irradiados con. Fibras de nucleoproteínas muy extendidas (COMPLEJO DNA +PROTEÍNAS= CROMOSOMAS). Puede ser: Heterocromatina o Eucromatina.
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Morphometric and ultrastructural studies of heterocrmoatina effect of infrared laser on rabbit temporo-mandibular joint fibroblasts. The infrared laser beams has been successfully used in joint lesions treatment due to its analgesic anti inflamatory and healing action, and on a cellular level, inducing an accentuated synthesis of ATP, activating cellular proliferation and collagen synthesis.
They were prepared for transmission electronic microscopy.
Transmission electronic micrographs of normal and irradiated fibroblasts were obtained with final increase of Volumetric fractions of the quoted cell corresponding to nucleus, cytoplasm, rough endoplasmatic reticule, eu and heterochromatin were evaluated.
In the same manner, the nucleus – cytoplasmatic heterocromstina and the area of each cellular type were quantified.
The results derived from the morphometric comparative study between the normal and irradiated fibroblasts indicated that there were significant differences with respect to the volumetric fractions of euchromatin and heterochromatin and fundamentally heterocromayina the cellular areas of both types. On the other hand, the rest of quantified parameters remained constant. It could be concluded that the cellular function reflected in collagen synthesis and secretion remained constant in both cellular types.
However, by means of infrared laser stimulations, this fibroblast facilitated protein synthesis, due to high percentage of transcriptionally active euchromatine. The susceptible cells, if stimulated by external effectors, constitute an excellent model for investigation of the biochemical, physiological, morphological and morphometric modifications that could characterize the process of cell euromatina.
In this context, the use of infrared laser treatment is useful in the temporo-mandibular joint treatment resulting an heterocrmatina effect Kim and Lee,healing and anti-inflammatory effect O” Kanes et al. In view of the eucromarina, it seemed important to study the morphological and morphometric differences observed between the temporo-mandibular joint disc fibroblasts pertaining to normal rabbits and those whose changes in their phenotype generated by a daily stimulation with infrared laser for a period of 10 consecutive days as the cause of modifications that would entail the alteration of its gene expression.
Considering that study of Cell Biology demonstrates that as long as the cells are stimulated, its components are subject to qualitative, quantitative and surface modifications, the analysis of electronic micrographs show how the modification of different components reflects into cellular function, pointing eucromaina the susceptible changes to be evaluated and compared between the normal and stimulated cells in relation to its morphological and morphometric aspects that determine the ultra-structural modifications pattern characterizing the radiation stimulation, in this biological model.
Junqueira and Salles, Starting with the discs pertaining to the temporomandibular joint of normal control rabbits and irradiated daily with infrared laser for a period of heeterocromatina consecutive days, samples were obtained for the investigation of fibroblasts. Subsequently, it was submitted to a wash solution of 6 g of NaC1 and 73 g of heterocromwtina dissolved in 1 l of j water.
The samples were studied and photographed in a Phillips EM electronic microscope. Beginning with the blocks for electronic microscopy, ultra fine slices were obtained in which each one of the cellular types were micrographed with an augmentation of For the evaluation of volumetric fractions of the different cellular components, a reticule of points was placed above the electronic micrographs and a differential count of the points having an incidence over the profiles of the cellular components took place, calculating the volumetric fraction occupied by a given component with the following equation: Volumetric fraction of the cellular heterorcomatina Pa: Points having an incidence over the component of the investigation Heterocromafina Total points having an incidence on the investigated cell.
In the electronic micrographs obtained from the normal as well as the irradiated fibroblasts Fig. The volumetric fractions occupied by the nucleus as well as the rough endoplasmatic reticule RER manifested in great heterodromatina cisterns were maintained constant in the normal fibroblasts as well as in the irradiated fibroblasts.
Results are in Figure 3. In the same manner, the nucleo-cytoplasmatic relation quantified in the normal fibroblasts as well as in those irradiated were maintained equally constant being equivalent to 0.
The volumetric fractions corresponding to the euchromatine increased, while those corresponding to the heterochromatin decreased from the normal eucromagina to those irradiated Fig. The cellular area pertaining to the irradiated fibroblasts showed an accentuated decrease, equivalent to 40 square microns in relation to the normal fibroblasts Table 1.
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The results from the analysis of the morphological data obtained with the transmission electronic microscopy fundamentally considering those relative to the similar volumetric fractions of cytoplasm, nucleus and those corresponding to the rough endoplasm reticule from the normal as heterocromatia as irradiated fibroblasts, demonstrated that these cells possessed similar morphological parameters, indicating that heterocgomatina effects produced by the stimulations with heterocromatian laser did not have an inhibiting action, nor an activating one of the cellular activity, situation which would translate in concluding primarily that these cells displayed a cellular function translated to a rate of protein synthesis-secretion practically similar.
These data differed from those described by Marques et al. The present results differed from those obtained by Chomette et al. In relation to the absence of intracellular collagen, the present results coincided with those of Berkovitz and Pacy obtained by studying the ultra structure of the fibro-cartilage also pertaining to the temporo-mandibular joint of the rabbit.
The present findings also coincided with those found by Pogrel et al. However, having the evaluation of increased euchromatine volumetric fractions in the irradiated fibroblasts as a base, thus with accentuated RNA transcription rates and its respective relation with the volume found in the rough reticulum, allowed an argument uecromatina the rate of collagen synthesis could increase, to a period of high metabolic activity, “to full function” as a response to the stimulations of infrared radiation in relation to that found in the normal fibroblasts.
The difference in the density of the cytoplasm observed in the electronic microscopy, as well as the decrease of the visualized cellular area in the irradiated fibroblasts, with respect to the normal ones, constituted an optimum representation of the effect that the infrared laser stimulations generated in this cell type. It would be pertinent to recall that this effect, a product of the radiation, decreased the area of the irradiated cell to one half, 82 to 40 square microns transforming it in the typical morphology of a “juvenile” fibroblast, making it tempting to suppose that this situation was the translation of a change in the gene expression modulated by these infrared stimulations.
Histoenzymology and electron microscopy study.
J Biol Buccale 15 1 Rio de Janeiro, Guanabara-Koogan, p. Gordon and Breach, London. Feasibility of wound dressing transillumination. J Clin Laser Med Surg. Laser in Surgery and Medicine 34 P; Matson, E; Marques, M. Pogrel, MA; Chen, J. Quintessence Int20 April 10, ; Revised: September 17, ; Accepted: All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License.
Services on Demand Journal. Stereological Method Beginning with the blocks for electronic microscopy, ultra fine slices were obtained in which each one of the cellular types were micrographed with an augmentation of Total points having an incidence on the investigated cell For the calculation of the cellular area, a Polar Ott Kempte, Bayern Germany Planimeter was used.
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